This study compared the activity profile of national and international female rugby sevens players during competitive matches. 20 rugby sevens female players were recruited, 10 were members of the Spanish National Team (26.27 ± 4.05 yr.old, 65.39 ± 5.01 kg, 166.72 ± 6.70 cm) and 10 were amateur athletes from a Spanish rugby championship (32.12 ± 6.40 yr.old, 66.48 ± 5.38 kg of body mass, 167.37 ± 3.02 cm). Data collection was conducted over four matches in each of two tournaments, national and international. Distance, velocity and heart rate were recorded using GPS devices for all participants throughout each match. There were significant differences (p<0.01) in total distance (1642 ± 171 vs 1363 ± 222m), average speed (6.0 ± 0.3 vs 5.2 ± 0.6 Km.h), number of sprints (6.1 ± 3.1 vs 1.9 ± 1.4 sprints) and distance covered in sprinting (118.8 ± 61.4 vs 47.0 ± 38.8 m). Significant differences were found at >95% HRmax, both for first (p <0.01) and second half (p <0.05). The work-rest ratio was significantly different (p<0.01) between international (1:0.3) and national players (1:0.4). Significant differences were found in accelerations above 2 ms in first and second half between the two groups. These findings suggest that distance covered in a match and speed is considerably different between international and national rugby sevens players and this is reflected as higher intensity of play with consequently higher heart rate. Coaches may use this information to design specific running drills for this athlete population to match the requirements of national or international game play.