Promoting healthy behaviors throughout life is an essential prevention tool. Prior research showed that unhealthy behaviors tend to co-occur and interplay. However, which behaviors co-occur most frequently and which sociodemographic variables are associated with specific clusters of unhealthy behavior are still being determined. This study aimed to identify different lifestyle profiles and analyze their associations with sociodemographic factors in an Italian academic community to plan targeted initiatives to promote healthy lifestyles. A sample of 8715 adults from an Italian university (mean age = 26 years; range = 18–76; 30% male) participated in an online survey in 2019. Four health-related behaviors were evaluated: diet, physical activity, smoking, and alcohol consumption. Lifestyle profiles were identified through cluster analysis. Then, a multinomial logistic regression was performed to explore the association among lifestyle profiles, sociodemographic variables (age, gender, and academic role), and body mass index (BMI). Results showed that older age was associated with the probability of belonging to the profile characterized by smoke addiction and regular alcohol consumption but also with the healthiest diet. The younger the age, the greater the probability of belonging to the most physically active profile. Men were more likely than women to belong to the lifestyle profile with the most regular alcohol consumption and the highest physical activity. Lower BMI was associated with the most physically active profile. This study shed light on factors associated with different co-occurring health-related behaviors that should be considered in planning effective communication strategies and preventive health interventions within the academic community.