The present paper aims to investigate the evolution of velocity fields as well as secondary flows in an open channel bend under the influence of negatively buoyant jets. A 135-degree open channel bend was used for experiments, and the jet nozzle was located along the outer bank in the straight section upstream of the bend. Efforts were made to specify the flow structures with high precision measurements of three-dimensional velocities by means of a three-dimensional PIV (Particle Image Velocimetry) technology. The experimental results show that the jets comparatively affect the flow structure at the beginning and exit of the flow in a bend. Although the jets had little effect on the maximum streamwise velocity, complex secondary flow patterns and properties were found to be influenced due to the occurrence of the negatively buoyant jets in the bend.