The direct quantification of plant biomarkers in sap is crucial to enhancing crop production. However, current approaches are inaccurate, involving the measurement of non-specific parameters such as colour intensity of leaves, or requiring highly invasive processes for the extraction of sap. In addition, these methods rely on bulky and expensive equipment, and they are time-consuming. The present work reports for the first time a low-cost sensing device that can be used for the simultaneous determination of sap K+ and pH in living plants by means of reverse iontophoresis. A screen-printed electrode was modified by deposition of a K+-selective membrane, achieving a super-Nernstian sensitivity of 70 mV Log[K+]−1 and a limit of detection within the micromolar level. In addition, the cathode material of the reverse iontophoresis device was modified by electrodeposition of RuOx particles. This electrode could be used for the direct extraction of ions from plant leaves and the amperometric determination of pH within the physiological range (pH 3–8), triggered by the selective reaction of RuOx with H+. A portable and low-cost (<£60) microcontroller-based device was additionally designed to enable its use in low-resource settings. The applicability of this system was demonstrated by measuring the changes in concentration of K+ and pH in tomato plants before and after watering with deionised water. These results represent a step forward in the design of affordable and non-invasive devices for the monitoring of key biomarkers in plants, with a plethora of applications in smart farming and precision agriculture among others.