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Retinal vessel segmentation is critical in detecting retinal blood vessels for a variety of eye disorders, and a consistent computerized method is required for automatic eye disorder screening. Many methods of retinal blood vessel segmentation are implemented, but these methods only yielded accuracy and lack of good sensitivity due to the coherence of retinal blood vessel segmentation. Another main factor of low sensitivity is the proper technique to handle the low-varying contrast problem. In this study, we proposed a five-step technique for assessing the impact of retinal blood vessel coherence on retinal blood vessel segmentation. The proposed technique for retinal blood vessels involved four steps and is known as the preprocessing module. These four stages of the pre-processing module handle the retinal image process in the first stage, uneven illumination and noise issues using morphological operations in the second stage, and image conversion to grayscale using principal component analysis (PCA) in the third step. The fourth step is the main step of contributing to the coherence of retinal blood vessels using anisotropic diffusion filtering and testing their different schemes and get a better coherent image on the optimized anisotropic diffusion filtering. The last step included double thresholds with morphological image reconstruction techniques to produce a segmented image of the vessel. The performances of the proposed method are validated on the publicly available database named DRIVE and STARE. Sensitivity values of 0.811 and 0.821 on STARE and DRIVE respectively meet and surpass other existing methods, and comparable accuracy values of 0.961 and 0.954 on STARE and DRIVE databases to existing methods. This proposed new method for retinal blood vessel segmentations can help medical experts diagnose eye disease and recommend treatment in a timely manner.