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Wiley, Journal of Applied Microbiology, 3(134), 2023

DOI: 10.1093/jambio/lxad020

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Flies (Insecta, Diptera) collected in the environment of dairy farms as carriers of Rotavirus A and betacoronavirus

Journal article published in 2023 by Amanda Bezerra Bertolini ORCID, Patrícia Jacqueline Thyssen ORCID, Paulo Eduardo Brandão, Aline Marrara Prado, Sheila Oliveira de Souza Silva, Mateus de Souza Ribeiro Mioni, Fábio Lucas Rezende de Gouvea, José Carlos de Figueiredo Pantoja, Helio Langoni, Felipe Freitas Guimarães, Sâmea Fernandes Joaquim ORCID, Simony Trevizan Guerra, Domingos da Silva Leite, Vera Mores Rall, Rodrigo Tavanelli Hernandes and other authors.
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

AbstractAimsWe aimed to investigate the prevalence of rotavirus and coronavirus in dipterans that commonly inhabit the environment of dairy farms.Methods and ResultsWe collected 217 insect specimens from nine dairy farms, which were examined through hemi-nested RT-PCR followed by Sanger sequencing in search of VP1 and N genes for rotavirus and bovine coronavirus-BCoV, respectively. With a predominance of Muscidae (152/217 = 70%) 11 families of Diptera were identified. Rotavirus A (RVA) and betacoronavirus (BCoV) were detected in 14.7% (32/217) and 4.6% (10/217) of the dipterans, respectively. Sequencing of the amplicons was possible for 11.5% (25/217) of RVA and 0.5% (1/217) of BCoV, confirming the presence of these pathogens.ConclusionsOur findings highlight the role of dipterans as carriers of RVA and BCoV of great relevance for public and animal health.