AbstractThis study investigated different amino acid-based surfactants (AASs), also known as biosurfactants, including sodium N-dodecyl asparagine (AS), sodium N-dodecyl tryptophan (TS), and sodium N-dodecyl histidine (HS) for their potential anticorrosion, antibacterial, and antidermatophyte properties. The chemical and electrochemical techniques were employed to examine the copper corrosion inhibition efficacy in H₂SO₄(1.0 M) solution at 298 K. The results indicated their promising corrosion inhibition efficiencies (% IEs), which varied with the biosurfactant structures and concentrations, and the concentrations of corrosive medium. Higher % IEs values were attributed to the surfactant adsorption on the copper surface and the production of a protective film. The adsorption was in agreement with Langmuir adsorption isotherm. The kinetics and mechanisms of copper corrosion and its inhibition by the examinedAASswere illuminated. The surfactants behaved as mixed-kind inhibitors with minor anodic priority. The values of % IEs gained from weight loss technique at a 500 ppm of the tested surfactants were set to be 81, 83 and 88 forAS, HSandTS, respectively. The values of % IEs acquired from all the applied techniques were almost consistent which were increased in the order:TS > HS ≥ AS, establishing the validity of this study. These surfactants also exhibited strong broad-spectrum activities against pathogenic Gram-negative and Gram-positive bacteria and dermatophytes.HSexhibited the highest antimicrobial activity followed byTS, andAS. The sensitivity of pathogenic bacteria varied against testedAASs.Shigella dysenteriaeandTrichophyton mantigrophyteswere found to be the most sensitive pathogens.HSexhibited the highest antibacterial activity againstShigella dysenteriae, Bacillus cereus, E. coli,K. pneumoniae,andS. aureusthrough the formation of clear zones of 70, 50, 40, 39, and 35 mm diameters, respectively.AASsalso exhibited strong antifungal activity against all the tested dermatophyte molds and fungi.HScaused the inhibition zones of 62, 57, 56, 48, and 36 mm diameters againstTrichophyton mantigrophytes,Trichophyton rubrum, Candida albicans, Trichosporon cataneum,andCryptococcus neoformans, respectively.AASsminimal lethal concentrations ranged between 16 to 128 µg/ml.HSpresented the lowest value (16 µg/ml) against tested pathogens followed byTS(64 µg/ml), andAS(128 µg/ml). Therefore,AASs, especiallyHS,could serve as an effective alternative antimicrobial agent against food-borne pathogenic bacteria and skin infections-associated dermatophyte fungi.