The Mediterranean basin constitutes one of the best areas to analyse Neanderthal populations and lifestyles in South-Western Europe. In this context, new excavations conducted in the Middle Palaeolithic site of Cova del Puntal del Gat expand the information available regarding this rich region. In this study, new results are reported, including detailed studies on stratigraphy, lithic technology, anthracology, carpology, and zooarchaeology and taphonomy of macro and micromammals, with the final objective of characterizing the Neanderthals’ subsistence strategies and occupational patterns. These results are framed within a broader regional study perspective that includes MIS 5 and 4 sites. Chronostratigraphic review has enabled us to reorganize many sites that were originally included in MIS 3, towards older stages belonging to the end of MIS 4 and throughout MIS 5.