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American Society for Microbiology, Applied and Environmental Microbiology, 6(79), p. 2096-2098, 2013

DOI: 10.1128/aem.03112-12

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Isolation of a Substantial Proportion of Forest Soil Bacterial Communities Detected via Pyrotag Sequencing

Journal article published in 2013 by David VanInsberghe, Gordon R. Stewart, Martin Hartmann ORCID, William W. Mohn
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT We isolated 1,264 bacterial strains from forest soils previously surveyed via pyrosequencing of rRNA gene amplicons. Conventional culturing techniques recovered a substantial proportion of the community, with isolates representing 22% of 98,557 total pyrotags. Growth characteristics of isolates indicated that ecological traits were associated with relative abundances of corresponding pyrotag operational taxonomic units.