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American Society for Microbiology, Antimicrobial Agents and Chemotherapy, 3(57), p. 1474-1479, 2013

DOI: 10.1128/aac.01275-12

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Rectal Swabs Are Suitable for Quantifying the Carriage Load of KPC-Producing Carbapenem-Resistant Enterobacteriaceae

Journal article published in 2013 by A. Lerner, J. Romano, I. Chmelnitsky, S. Navon-Venezia ORCID, R. Edgar, Y. Carmeli
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT It is more convenient and practical to collect rectal swabs than stool specimens to study carriage of colon pathogens. In this study, we examined the ability to use rectal swabs rather than stool specimens to quantify Klebsiella pneumoniae carbapenemase (KPC)-producing carbapenem-resistant Enterobacteriaceae (CRE). We used a quantitative real-time PCR (qPCR) assay to determine the concentration of the bla KPC gene relative to the concentration of 16S rRNA genes and a quantitative culture-based method to quantify CRE relative to total aerobic bacteria. Our results demonstrated that rectal swabs are suitable for quantifying the concentration of KPC-producing CRE and that qPCR showed higher correlation between rectal swabs and stool specimens than the culture-based method.