AbstractToday, only few ready‐to‐use and convenient decision‐making tools are available in ecotoxicology concerning accumulation and effects of chemical substances on organisms, accounting for exposure situations that are known to be complex (routes of exposure, metabolism, mixtures, etc.). This paper presents new perspectives on the generic calculation of bioaccumulation metrics via the innovative web tool MOSAIC_bioacc (http://mosaic.univ‐lyon1.fr/bioacc). MOSAIC_bioacc provides all kinds of bioaccumulation metrics associated with their uncertainty whatever the species‐compound combination. MOSAIC_bioacc expects accumulation‐depuration data as inputs, even with complex exposure and clearance patterns, to quickly perform their relevant analysis. MOSAIC_bioacc intends to facilitate the daily work of regulators, or any ecotoxicologist, who will freely benefit from a user‐friendly online interface that automatically fits toxicokinetic models without need for users to invest in the technical aspects to get bioaccumulation metrics estimates. MOSAIC_bioacc also provides all results in a fully transparent way to ensure reproducibility. Integr Environ Assess Manag 2022;18:10–18. © 2021 SETAC