Abstract Two-dimensional (2D) magnetic materials have developed rapidly in recent years, which exhibited a lot of novel and interesting properties, such as the topological Hall effect, magnetic skyrmions and charge density waves. Most hitherto known 2D antiferromagnetic materials are insulating, while 2D antiferromagnets with intrinsic metallicity are rarely reported. Hindered by such shortage, the electrical properties of the metallic 2D antiferromagnetic materials remain largely unexplored. Here we report high-quality synthesis and comprehensive characterization of a van der Waals antiferromagnetic metal, V₅Se₈, and observation of an anomalous Hall effect in magneto-transport measurements. The temperature dependence of longitudinal-resistance reveals a metallic behavior in the as-grown V₅Se₈ flakes. The transversal resistance measurements reveal a non-zero Hall resistance with two antisymmetric peaks, which is attributed to a non-zero Berry curvature. Our work demonstrates the novel property of 2D metallic antiferromagnetic material V₅Se₈ for the application of 2D spintronics.