AbstractSynthesized micro‐ and nanotubes composed of transition metal dichalcogenides (TMDCs) such as MoS₂ are promising for many applications in nanophotonics because they combine the abilities to emit strong exciton luminescence and to act as whispering gallery microcavities even at room temperature. In addition to tubes in the form of hollow cylinders, there is an insufficiently studied class of twisted tubes, the flattened cross‐section of which rotates along the tube axis. As shown by theoretical analysis, in such nanotubes the interaction of electromagnetic waves excited at the opposite sides of the cross‐section can cause splitting of the whispering gallery modes. By studying micro‐photoluminescence spectra measured along individual MoS₂ tubes, it has been established that the splitting value, which controls the energies of the split modes, depends exponentially on the aspect ratio of the cross‐section, which varies in “breathing” tubes, while the relative intensity of the modes in a pair is determined by the angle of rotation of the cross‐section. These results open up the possibility of creating multifunctional tubular TMDC nanodevices that provide resonant amplification of self‐emitting light at adjustable frequencies.