AbstractCopper isotopes (δ⁶⁵Cu) in hydrothermal fluids have the potential to provide information on ore‐forming processes occurring below the seafloor, but Cu isotope data from high‐temperature fluids are scarce. Here, we examine the extent to which coexisting sulfide minerals in a hydrothermal chimney can preserve fluid Cu isotope ratios using a fluid‐solid pair of a black smoker (333°C) from the Roman Ruins vent area (PACMANUS) in the Manus Basin. Two ca. 3 cm long transects through the chalcopyrite‐rich chimney wall show an increase in δ⁶⁵Cu from 0.48 to 2.28‰ from the interior to the exterior, coupled with limited variation in sulfide δ³⁴S (1.52–4.72‰). The Cu isotopic composition of chalcopyrite from the innermost wall closely resembles the δ⁶⁵Cu value of the paired hydrothermal fluid, indicating that chalcopyrite in the inner ∼5 mm of the chimney records the Cu isotope ratio of the venting fluid. Beyond this, an increase in sulfide δ⁶⁵Cu toward the exterior correlates with an increase in the relative abundance of secondary Cu sulfides. The appearance of bornite coincides with the presence of small barite crystals, suggesting this represents a redox gradient between reduced hydrothermal fluids and oxidized seawater admixing inwards. Elevated δ⁶⁵Cu in this zone can be explained by the precipitation of secondary Cu sulfides from ⁶⁵Cu‐enriched fluids formed during oxidative chalcopyrite dissolution. Our findings indicate that interactions with oxidizing seawater shift chalcopyrite δ⁶⁵Cu values over small spatial scales, and that caution must be applied if chimney sulfides are used to reconstruct δ⁶⁵Cu values of high‐temperature hydrothermal fluids.