AbstractAtmospheric inversion estimates of net ecosystem exchange (NEE) of CO₂ are increasingly relevant to climate policy. We evaluated sub‐continental, seasonal estimates of CO₂ NEE from nine global inversion systems that participated in the Orbiting Carbon Observatory‐2 model intercomparison project (OCO‐2 v9 MIP), using 98 research flights conducted over the central and eastern United States from 2016 to 2018 as part of the Atmospheric Carbon and Transport ‐ America mission. We found that the seasonal amplitude of NEE in the central and eastern United States is underestimated in these models and model‐data biases are largest for those inversions with the smallest seasonal flux amplitudes. These results were independent of whether the inversions used satellite or in situ data. The largest NEE biases were observed in the Midwest croplands and eastern forests. Future experiments are needed to determine the causes of the persistent biases and if they are associated with biases in annual flux estimates.