Building machine learning (ML) calibrations using near-infrared (NIR) soil spectroscopy direct in agricultural areas (online NIR), soil attributes can be fine-scale mapped in a faster and more cost-effective manner, guiding management decisions to ensure the maintenance of soil functions. However, a financially and environmentally unattractive density of 3–5 laboratory soil samples per ha is required to build these calibrations. Since no reports have evaluated if they are reusable or if a new calibration is required for each acquisition, this study’s objective was to acquire online NIR spectra in an agricultural field where ML models were previously built and validated, assessing their performance over time. Two spectral acquisitions were held over a fallow tropical field, separated by 21 days. Soil properties (clay, organic matter, cation exchange capacity, pH, phosphorus, potassium, calcium, and magnesium) were predicted using principal components regression models calibrated with day 1 spectra. Day 1 and day 21 predicted values and maps interpolated by ordinary kriging were compared. Spectra characteristics (morphology, features, and intensity) were evaluated. Predicted values from the two days were not correlated, as no causal relationship was found for the only Pearson’s correlation coefficient (r) significative at 99% (p < 0.01) (calcium, with r = 0.22 in the comparison pairing the nearest neighbors from the two days). For clay, organic matter, and cation exchange capacity, despite their robust prediction on day 1, no significative r values were found, ranging from −0.14 to 0.32, when comparing day 1 with day 21. The maps of the two days presented no similar spatial distribution, hindering their use for management decisions. Soil moisture is a suggested source of variation, but the analysis indicated that it was not the only one, requiring further investigation of the effect of soil surface conditions and environmental variables. Although further investigations should be performed, the results presented suggest that online NIR spectra ML models require spatio-temporal local calibrations to perform properly.