Abstract Different parameters are used when comparing SERS substrates in the research of most scientific groups. These parameters are more often aimed at comparing theory and experiment. We described experimental models for comparison and explain their applicability. In this work, a reaction mixture volume fixing technique was chosen when comparing different substrates. The intensity distribution maps with an area of 2025 μm²(9X9 points) were recorded and described. We successfully used the parameters average intensity of intensity distribution maps and percentage gate of intensity distribution maps for comparison of enhancing substrate. We have observed uniform enhancing across the entire surface of self-assembled plasmonic film. A comparative study has shown that the self-assembled plasmonic film sample has high enhancing factors and good reproducibility of factors over the studying sample surface. Average intensity of dropcasted colloidal gold nanoparticles (Au-NPs) sample is equal to 1860 counts and percentage gate of intensity data equals 125%. Average intensity of self-assembling plasmonic film is equal to 112000 counts and percentage gate equals 1,67%.