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MDPI, Viruses, 6(15), p. 1356, 2023

DOI: 10.3390/v15061356

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Detection of Zika Virus in Aedes aegypti and Aedes albopictus Mosquitoes Collected in Urban Forest Fragments in the Brazilian Amazon

Journal article published in 2023 by Erika Oliveira Gomes ORCID, Lívia Sacchetto ORCID, Maurício Teixeira ORCID, Bárbara Aparecida Chaves ORCID, Adam Hendy ORCID, Claudia Mendonça, Izabele Guimarães, Ramon Linhares, Daniela Brito, Danielle Valério, Jady Shayenne Mota Cordeiro ORCID, Alexandre Vilhena Silva Neto, Vanderson Souza Sampaio ORCID, Vera Margarete Scarpassa ORCID, Michaela Buenemann ORCID and other authors.
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Zika virus (ZIKV) is an RNA flavivirus (Flaviviridae family) endemic in tropical and subtropical regions that is transmitted to humans by Aedes (Stegomyia) species mosquitoes. The two main urban vectors of ZIKV are Aedes aegypti and Aedes albopictus, which can be found throughout Brazil. This study investigated ZIKV infection in mosquito species sampled from urban forest fragments in Manaus (Brazilian Amazon). A total of 905 non-engorged female Ae. aegypti (22 specimens) and Ae. albopictus (883 specimens) were collected using BG-Sentinel traps, entomological hand nets, and Prokopack aspirators during the rainy and dry seasons between 2018 and 2021. All pools were macerated and used to inoculate C6/36 culture cells. Overall, 3/20 (15%) Ae. aegypti and 5/241 (2%) Ae. albopictus pools screened using RT-qPCR were positive for ZIKV. No supernatants from Ae. aegypti were positive for ZIKV (0%), and 15 out of 241 (6.2%) Ae. albopictus pools were positive. In this study, we provide the first-ever evidence of Ae. albopictus naturally infected with ZIKV in the Amazon region.