International Union of Crystallography, Acta Crystallographica. Section d, Structural Biology, 6(77), p. 835-839, 2021
DOI: 10.1107/s2059798321002291
Full text: Unavailable
Principal component analysis (PCA) has been widely proposed to analyze flexibility and heterogeneity in cryo-electron microscopy (cryoEM). In this paper, it is argued that (i) PCA is an excellent technique to describe continuous flexibility at low resolution (but not so much at high resolution) and (ii) PCA components should be analyzed in a concerted manner (and not independently).