Introduction Awareness of screening procedures and illness warning signals is critical for expanding and implementing screening programs in society, which would improve the odds of early identification of breast cancer. Objectives This study aimed to evaluate the knowledge, awareness, attitudes, and practices related to breast cancer risk factors, signs, symptoms and methods of screening among female faculty and students at Hail University in the Kingdom of Saudi Arabia. Methods A cross-sectional study was conducted from January 2021 through February 2021 in the Hail region of Saudi Arabia. A closed-ended questionnaire, which consisted of 37 questions, was distributed online (using a Google Forms link) in both English and Arabic languages. Data was collected from 425 female subjects who participated in the study. Results The study showed an overall knowledge level of 46.36% regarding breast cancer. Participants had average knowledge about risk factors, signs, and symptoms, whereas their awareness and practice of breast self-examination and screening methods were weak. Conclusion The current study concluded that public awareness of breast cancer remains relatively low, and Saudi Arabia still needs several public awareness initiatives using mass media, such as television, the Internet, and radio, as well as social media. Special awareness programs should also be held in places where a large number of women can easily be reached, such as colleges, universities, and hospitals.