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American Society for Microbiology, Applied and Environmental Microbiology, 10(87), 2021

DOI: 10.1128/aem.02690-20

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Novel 3- O -α- d -Galactosyl-α- l -Arabinofuranosidase for the Assimilation of Gum Arabic Arabinogalactan Protein in Bifidobacterium longum subsp. longum

Journal article published in 2021 by Yuki Sasaki, Ayako Horigome, Toshitaka Odamaki ORCID, Jin-Zhong Xiao, Akihiro Ishiwata ORCID, Yukishige Ito ORCID, Kanefumi Kitahara, Kiyotaka Fujita ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Bifidobacteria harbor numerous carbohydrate-active enzymes that degrade several dietary fibers in the gastrointestinal tract. B. longum JCM7052 is known to exhibit the ability to assimilate gum arabic AGP, but the key enzyme involved in the degradation of gum arabic AGP remains unidentified. Here, we cloned and characterized a GH39 3- O -α- d -galactosyl-α- l -arabinofuranosidase (GAfase) from B. longum JCM7052.