AbstractBackgroundChukrasia velutina is an enthnomedicinally used plant reported to have significant medicinal values. The present study aimed to explore the pharmacological activities of bark methanol extract using in vitro, in vivo and in silico models.MethodsThe study was designed to investigate the pharmacological effects of methanol extract of Chukrasia velutina bark (MECVB) through in vitro, in vivo and in silico assays. Analgesic activity was tested using formalin‐induced nociception and acetic acid‐induced writhing assays while the antipyretic effect was tested using yeast‐induced hyperthermia in mice model. The antioxidant effect was tested using the DPPH and reducing power assay and the cytotoxic screening was tested using the brine shrimp lethality bioassay. In addition, in silico studies were conducted using computer aided methods.ResultsIn the acetic acid‐induced writhing assay, the extract showed 28.36% and 56.16% inhibition of writhing for doses of 200 and 400 mg/kg, respectively. Moreover, a dose‐dependent formalin‐induced licking response was observed in both early and late phase. In yeast‐induced pyrexia, the MECVB exhibited (p < 0.05) antipyretic effect. The extract demonstrated an IC₅₀ value of 78.86 μg/ml compared with ascorbic acid (IC₅₀ 23.53 μg/ml) in the DPPH scavenging assay. The compounds sitosterol, 5,7‐dimethoxycoumarin and scopoletin were seen be effective in molecular docking scores against COX‐I (2OYE), COX‐II (6COX) and human peroxiredoxin 5 (1HD2). In ADME/T analysis, 5,7‐dimethoxycoumarin and scopoletin satisfied Lipinski's rule of five and thus are potential drug candidates.ConclusionThe bark of Chukrasia velutina showed significant analgesic and antipyretic properties and is a potential source of natural anti‐oxidative agents.