AbstractHypotheses regarding favorable conditions for anomalous charging have primarily resulted from studies within the Great Plains region of the United States, where the efficiency of warm precipitation processes is thought to be fundamental. Rare observations of anomalous charge structures in the Southeastern region challenge existing conceptual models used to explain anomalous charging. As a rigorous evaluation of conditions that support anomalous charge structures, environmental characteristics and bulk kinematic and microphysical properties of two normal and two anomalous supercell thunderstorms observed in the Southeast were compared. Within the anomalous supercells, greater quantities of precipitation ice were identified at higher altitudes and colder temperatures, suggesting a greater depth of riming growth and increased vertical transport of rimed hydrometeors. Deeper anomalous supercell updrafts were larger and stronger in the upper mixed‐phase and glaciated regions. However, normal supercells were characterized by more robust low‐level updrafts, resulting in comparable warm cloud residence times that suggested warm precipitation processes were not necessarily less efficient in the anomalous supercells. Indications of enhanced mixed‐phase liquid water content in favor of anomalous charging were observed in the anomalous supercells, though contrasts in related environmental parameters were not as large as observed in other comparative studies. Anomalous supercell environments were characterized by increased instability, shallower warm cloud depth, as well as lower relative humidity in the 700–500 mb layer. Evidence of impacts from dry air in anomalous storm structures suggested that water vapor content may have affected particle‐scale charge transfer in support of anomalous charge structure development.