This paper presents a multi-instrument observational analysis of the equatorial plasma bubbles (EPBs) variation over the American sector during a geomagnetically quiet time period of 07–10 December 2019. The day-to-day variability of EPBs and their underlying drivers are investigated through coordinately utilizing the Global-scale Observations of Limb and Disk (GOLD) ultraviolet images, the Ionospheric Connection Explorer (ICON) in-situ and remote sensing data, the global navigation satellite system (GNSS) total electron content (TEC) observations, as well as ionosonde measurements. The main results are as follows: 1) The postsunset EPBs’ intensity exhibited a large day-to-day variation in the same UT intervals, which was fairly noticeable in the evening of December 07, yet considerably suppressed on December 08 and 09, and then dramatically revived and enhanced on December 10. 2) The postsunset linear Rayleigh-Taylor instability growth rate exhibited a different variation pattern. It had a relatively modest peak value on December 07 and 08, yet a larger peak value on December 09 and 10. There was a 2-h time lag of the growth rate peak time in the evening of December 09 from other nights. This analysis did not show an exact one-to-one relationship between the peak growth rate and the observed EPBs intensity. 3) The EPBs’ day-to-day variation has a better agreement with that of traveling ionospheric disturbances and atmospheric gravity waves signatures, which exhibited relatively strong wavelike perturbations preceding/accompanying the observed EPBs on December 07 and 10 yet relatively weak fluctuations on December 08 and 09. These coordinate observations indicate that the initial wavelike seeding perturbations associated with AGWs, together with the catalyzing factor of the instability growth rate, collectively played important roles to modulate the day-to-day variation of EPBs. A strong seeding perturbation could effectively compensate for a moderate strength of Rayleigh-Taylor instability growth rate and therefore their combined effect could facilitate EPB development. Lacking proper seeding perturbations would make it a more inefficient process for the development of EPBs, especially with a delayed peak value of Rayleigh-Taylor instability growth rate.