The ophiolites in the Beishan Orogenic Belt provide important information about the evolution of the Beishan Ocean in the Paleozoic Era. We studied ophiolite petrology, geochemistry and isotopic chronology. The Shazouquan ophiolites consist of dunites, wehrlites, gabbros and anorthosites. Ophiolitic mélange belts are composed of matrixes and blocks, and different rocks are fault-bounded. Dunites and wehrlites are high in Mg#, Cr# and MgO, low in TiO2, relatively depleted in large-ion lithophile elements (Ti and P) and enriched in high-strength elements (U, Zr and Hf). They have a total REE of 1.25 × 10–6−5.39 × 10−6 and δEu of 1.12–3.54, which are similar to those of SSZ-type ophiolites, indicating that their parent magma source region may be a weakly depleted mantle source region. The anorthosite and gabbro are high in Al2O3, MgO and Mg#, low in TiO2, enriched in large-ion lithophile elements (Rb and Sr), and depleted in high-strength elements (Nb, Ta and Ti), but enriched in Zr and Hf. They have similar geochemical signatures to those of arc magmatic rocks. They are derived from the mantle peridotite formed against the tectonic background of subduction and modified by the fluid materials in the subduction zone. We collected anorthosite and gabbro, which were produced as ophiolite for U-Pb dating. The anorthosite yields a zircon U-Pb, aged 394 ± 11 Ma (MSWD = 0.84), and a gabbro zircon U-Pb, aged 466 ± 12 Ma (MSWD = 3.2), indicating that the Shazouquan ophiolite was formed in the Middle Ordovician–Early Devonian eras. Combining the above evidence, we conclude that the Beishan Ocean was in a subduction tectonic background from the Middle Ordovician to Early Devonian periods.