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Nature Research, Nature Methods, 2023

DOI: 10.1038/s41592-023-01846-7

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Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging

Journal article published in 2023 by Ellen M. Quardokus ORCID, Diane C. Saunders ORCID, Elizabeth McDonough ORCID, John W. Hickey ORCID, Christopher Werlein ORCID, Christine Surrette, Presha Rajbhandari, Anna Martinez Casals, Hua Tian ORCID, Lisa Lowery, Elizabeth K. Neumann, Frida Björklund, Taruna V. Neelakantan, Josh Croteau ORCID, Anne E. Wiblin ORCID and other authors.
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

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Abstract

AbstractMultiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas.