American Society for Microbiology, Microbiology Spectrum, 3(11), 2023
DOI: 10.1128/spectrum.03954-22
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Multilocus sequence typing (MLST) is the reference method for Listeria monocytogenes typing but is expensive and takes time to perform, from 3 to 5 days for laboratories that outsource sequencing. Thirty major MLST clonal complexes (CCs) are circulating in the food chain and are currently identifiable only by sequencing.