The present study aimed to evaluate the application of shear strength and its correlations with leaf blade morphological and chemical variables as a tool capable of detecting differences between species and accessions of the genus Paspalum in the early stages of a forage plant breeding programme. A total of 13 Paspalum accessions from two species (Paspalum atratum and Paspalum regnellii) were subjected to analyses of shear strength, which also analysed the neutral detergent fibre, acid detergent fibre, lignin, and digestibility. Morphological measurements were also performed to standardize the shear strength per unit of leaf tissue. The experimental design consisted of complete randomized blocks, with four replications and eight cuts performed over 2 years. Shear strength was evaluated for its potential usefulness for detecting differences between accessions of the same species and the correlations of accessions. It was possible to use the shear strength measured with the texturometer to detect differences between species and accessions of the Paspalum genus. The results showed significant correlations of shear strength and neutral detergent fibre (r = 0.49), and negative correlations were found between digestibility and shear strength (r = -0.55). These correlations were maintained when the strength variables were standardized according to the morphological variables. Thus, shear strength can be used in the initial stages of the selection of species of the genus Paspalum.