Such topological spin textures as magnetic skyrmions and antiskyrmions have attracted significant interest in recent years owing to their rich variety of underlying physics and potential applications in next-generation magnetic devices. In the domain of applications, it is essential to stabilize the topological spin textures over a wide range of temperatures, including room temperature, and manipulate them with various external stimuli. Significant developments have been made in room-temperature skyrmions and antiskyrmions arising from the Dzyaloshinskii–Moriya interaction (DMI) in several magnetic materials with broken inversion symmetry. In this Perspective, we review recent progress in non-centrosymmetric magnets with bulk DMI, which host skyrmions and antiskyrmions above room temperature. We first provide an overview of room-temperature Bloch-type skyrmions and the robustness of their metastability, the variety of their forms, and their dynamics in Co–Zn–Mn alloys with a β-Mn-type chiral structure. We then focus on room-temperature antiskyrmions as well as their topological transformations in Heusler alloys with D_2d symmetry and Pd-doped (Fe,Ni)₃P with S₄ symmetry. The robust skyrmions and antiskyrmions, with versatile tunability in these non-centrosymmetric materials at room temperature, represent a step toward the long-sought milestone of “skyrmionics.”