AbstractReducing environmental impacts in transport motivates many studies to offer more sustainable freight services. However, most methodologies focus on impacts from fuel consumption, and approaches trying to integrate other transport components have not facilitated its application to actual and specific transport services. In this study, we present a harmonized approach to address the transport services with a holistic way to increase the knowledge about hotspots of the transport sector based on the life cycle assessment methodology. In this framework, vehicle manufacturing, fuel production, and infrastructure construction are the key transport components around the traffic process. Besides fuel usage, the operation and maintenance of vehicles and infrastructures are also included. We developed a tool to create the life cycle inventories for each transport component to be applied to specific transport services in any location with a comprehensive view and low uncertainty in the results. This approach was applied to road-freight services in Colombia, Malaysia, and Spain. The main results showed the nature and origin of the environmental impacts, which are highly influenced by the emissions control technologies, road characteristics, and traffic volume. The contribution of atmospheric pollutants per tonne-km can decrease by a quarter when Euro VI trucks on highways instead of conventional trucks on single-lane roads are used. However, these contributions are highly affected by fuel production due to the origin of biofuels. The proposed methodology provides relevant information to estimate transport impacts in the life cycle assessment of products with superior precision and identify strategies for systemically improving sustainability.