Published in
American Society for Microbiology, mSystems, 5(6), 2021
DOI: 10.1128/msystems.00813-21
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Transcriptional and Post-Transcriptional Polar Effects in Bacterial Gene Deletion Libraries
Journal article published in 2021 by
André Mateus 
,
Malay Shah,
Johannes Hevler,
Nils Kurzawa,
Jacob Bobonis ,
Athanasios Typas,
Mikhail M. Savitski
,
Malay Shah,
Johannes Hevler,
Nils Kurzawa,
Jacob Bobonis ,
Athanasios Typas,
Mikhail M. Savitski
This paper is made freely available by the publisher.
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Abstract
Single-gene deletion libraries have allowed genome-wide characterization of gene function and interactions. While each mutant intends to disrupt the function of a single gene, it can unintentionally target other genes, such as those located in the same operon as the deletion.