Most land surface models can, depending on the simulation experiment, calculate the vegetation distribution and dynamics internally by making use of biogeographical principles or use vegetation maps to prescribe spatial and temporal changes in vegetation distribution. Irrespective of whether vegetation dynamics are simulated or prescribed, it is not practical to represent vegetation across the globe at the species level because of its daunting diversity. This issue can be circumvented by making use of 5 to 20 plant functional types (PFTs) by assuming that all species within a single functional type show identical land–atmosphere interactions irrespective of their geographical location. In this study, we hypothesize that remote-sensing-based assessments of aboveground biomass can be used to constrain the process in which real-world vegetation is discretized in PFT maps. Remotely sensed biomass estimates for Africa were used in a Bayesian framework to estimate the probability density distributions of woody, herbaceous and bare soil fractions for the 15 land cover classes, according to the United Nations Land Cover Classification System (UN-LCCS) typology, present in Africa. Subsequently, the 2.5th and 97.5th percentiles of the probability density distributions were used to create 2.5 % and 97.5 % credible interval PFT maps. Finally, the original and constrained PFT maps were used to drive biomass and albedo simulations with the Organising Carbon and Hydrology In Dynamic Ecosystems (ORCHIDEE) model. This study demonstrates that remotely sensed biomass data can be used to better constrain the share of dense forest PFTs but that additional information on bare soil fraction is required to constrain the share of herbaceous PFTs. Even though considerable uncertainties remain, using remotely sensed biomass data enhances the objectivity and reproducibility of the process by reducing the dependency on expert knowledge and allows assessing and reporting the credible interval of the PFT maps which could be used to benchmark future developments.