AbstractThe conflict between free-ranging livestock and wildlife is a serious conservation concern across rural communities worldwide. Livestock may affect wild herbivores via direct competition for resources due to spatial and diet overlap or via behavioural interference. It is imperative that we disentangle the effects of livestock on wildlife behaviour to obtain an empirical basis able to stir management and conservation decisions. Here, we studied the effect of livestock presence on the habitat selection in a free-ranging European mouflon (Ovis aries musimon) population in Sardinia, where the species is under strict protection. We collected spatial data on mouflon and livestock during two consecutive years to investigate whether the mouflon selection of key feeding grassland sites was negatively impacted by the livestock presence. We found that mouflon preferably selected grassland, and its selection significantly increased when grass was of better quality (greener). We showed that livestock presence led to the displacement of mouflon from such preferred feeding sites, an effect clearly exacerbated by livestock proximity. We indeed found that the selection of grassland by mouflon dropped significantly when the distance between livestock and mouflon was below ~ 650 m, providing a useful management threshold indication. Livestock presence in close proximity displaced mouflon to sub-optimal habitat, and its effects may have negative impact on the population dynamic of this species which is already characterized by low female productivity within harsh Mediterranean environment. Our results give clear management indications aimed at better managing livestock grazing within natural areas to ultimately improve wildlife conservation.