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Royal Society of Chemistry, Chemical Science, 19(12), p. 6569-6579, 2021

DOI: 10.1039/d1sc00364j

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Stepwise nitrosylation of the nonheme iron site in an engineered azurin and a molecular basis for nitric oxide signaling mediated by nonheme iron proteins

Journal article published in 2021 by Shiliang Tian ORCID, Ruixi Fan ORCID, Therese Albert ORCID, Rahul L. Khade ORCID, Huiguang Dai ORCID, Kevin A. Harnden, Parisa Hosseinzadeh, Jing Liu, Mark J. Nilges, Yong Zhang ORCID, Pierre Moënne-Loccoz ORCID, Yisong Guo ORCID, Yi Lu ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Stepwise nitrosylation from Fe(ii) to {FeNO}7, {FeNO}8and then to {Fe(NO)2}9is reported for the first time in the same protein scaffold, providing deeper understanding of the detailed mechanism of dinitrosyl iron complex formation.