Although most variation regarding bat colouration has been recorded for fur, there have also been a few bat species for which intraspecific variation in wing translucency has been reported. However, all records are from the Paleotropics, with no cases reported for any bat species in the Neotropics. Here we describe the first case of intraspecific variation in wing translucency for a Neotropical bat species, the lesser ghost bat (Diclidurus scutatus). Two individuals captured hundreds of kilometres away from each other in the Brazilian Amazon forest showed distinct patterns in relation to the degree of their wing translucency. While one individual in the North‐eastern Amazon forest had fully translucent wings, the other in the Southern Amazon had whitish opaque wings. We propose three hypotheses to explain this variation, which are related to differences in: (1) light conditions and camouflage in the roost; (2) thermoregulation requirements; and (3) habitat structure relationship with hunting success