ABSTRACT Objective: to validate the Knowledge, Attitude and Practice survey on Nursing assistance during delivery and childbirth based on the concepts of Florence Nightingale's Environmental Theory and the recommendations of the National Guidelines for Assistance to Normal Delivery. Method: this is a methodological study in which the Knowledge, Attitude and Practice survey was elaborated, and validated regarding content and appearance. Data collection was carried out between the months of July and December 2017. The validations were performed by 22 nurses who work in delivery and childbirth care and the data were analyzed using the Content Validity Index and the Binomial test. Results: content and face validation evidenced that the agreement among the experts was satisfactory (Content Validity Index greater than 0.80 for all items evaluated). Conclusion: it is concluded that the survey can be used to assess the nurses' Knowledge, Attitude and Practice in relation to the Nursing assistance provided during delivery and childbirth.