Published in
American Society for Microbiology, mSystems, 3(6), 2021
DOI: 10.1128/msystems.00619-21
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DNA Extraction and Host Depletion Methods Significantly Impact and Potentially Bias Bacterial Detection in a Biological Fluid
Journal article published in 2021 by
Erika Ganda 
,
Kristen L. Beck ,
Niina Haiminen ,
Justin D. Silverman ,
Ban Kawas,
Brittany D. Cronk ,
Renee R. Anderson,
Laura B. Goodman ,
Martin Wiedmann
,
Kristen L. Beck ,
Niina Haiminen ,
Justin D. Silverman ,
Ban Kawas,
Brittany D. Cronk ,
Renee R. Anderson,
Laura B. Goodman ,
Martin Wiedmann
This paper is made freely available by the publisher.
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Abstract
Tracking the bacterial communities present in our food has the potential to inform food safety and product origin. To do so, the entire genetic material present in a sample is extracted using chemical methods or commercially available kits and sequenced using next-generation platforms to provide a snapshot of the microbial composition.