For citrus, there is no definition of the ideal fruit harvesting point for seed extraction. Some studies have shown the positive effect of postharvest refrigerated storage of fruits to obtain quality seeds. This study aimed to evaluate the influence of the fruit maturation stage on the germination of Swingle citrumelo (CS) seeds after the refrigerated storage of fruits and seeds. CS fruits were harvested at 180 days after anthesis (DAA); 210 DAA and 240 DAA. Fruits and seeds were stored in cold chamber for 0, 15, 30, and 45 days. Physicochemical analyses were performed on fruits (colour, soluble solids, acidity and ratio) and seeds (water content, germination, and emergence). Germination of seeds extracted from fruits harvested at 180, 210, and 240 DAA, without refrigerated storage, showed a positive correlation with colour index. The refrigerated storage of fruits and seeds extracted from fruits harvested at 210 DAA, increased the seed germination rate. Peel colour (IC) was correlated positively with the germination of Swingle citrumelo seeds. The results of this study revealed that the absence of refrigerated storage, harvesting Swingle citrumelo fruits, with ripe characteristics (end of harvest), results in a high seed germination rate. On the other hand, refrigerated storage of fruits and seeds extracted from fruits harvested at mid-harvest increases the seed germination rate.