Background: The aim of this study was to evaluate the feasibility of a dual-bolus protocol, where a first bolus of an intravascular tracer is used to measure perfusion, followed by a second bolus of a freely filtered gadolinium-containing agent to measure fil-tration capacity. Methods: The study was conducted in mice subjected to complete unilateral ureteral obstruction (UUO), and sham operated mice were used as controls. Dynamic contrast-enhanced MRI was performed 2 days after surgery. Results and discussions: Mean signal-time curves of the renal cortex, renal medulla and abdominal aorta were used to calculate the relative renal blood flow (rRBF), relative renal blood volume (rRBV), mean transit time (MTT) and the glomerular transfer rate K trans . We demonstrated that kidneys suffering from two days of UUO showed a decrease in cortical as well as medullary rRBF compared to kidneys from sham -operated mice. Further, we found no changes in rRBV and MTT among groups, neither in the cor-tex nor in the medulla. The renal functional pa-rameter K trans showed a tendency (but statistically insignificant) to be reduced in the obstructed kid-ney compared to the sham-operated mice. Conclu-sions: We showed our first experiences with the consecutive use of intra-and extravascularly dis-tributed agents in a renal-diseased mouse model, allowing analysis of both functional haemo-dyamics and filtration capacity in kidneys.