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American Society for Microbiology, Journal of Clinical Microbiology, 6(59), 2021

DOI: 10.1128/jcm.03271-20

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Diagnosis of SARS-CoV-2 Infection with LamPORE, a High-Throughput Platform Combining Loop-Mediated Isothermal Amplification and Nanopore Sequencing

Journal article published in 2021 by Leon Peto ORCID, Gillian Rodger, Daniel P. Carter ORCID, Karen L. Osman, Mehmet Yavuz, Katie Johnson, Mohammad Raza, Matthew D. Parker, Matthew D. Wyles, Monique Andersson, Anita Justice, Alison Vaughan, Sarah Hoosdally, Nicole Stoesser ORCID, Philippa C. Matthews ORCID and other authors.
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

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Abstract

LamPORE is a novel diagnostic platform for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA combining loop-mediated isothermal amplification with Nanopore sequencing, which could potentially be used to analyze thousands of samples per day on a single instrument. We evaluated the performance of LamPORE against reverse transcriptase PCR (RT-PCR) using RNA extracted from spiked respiratory samples and stored nose and throat swabs collected at two UK hospitals.