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American Society for Microbiology, Journal of Clinical Microbiology, 7(59), 2021

DOI: 10.1128/jcm.02889-20

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A Whole-Genome-Based Gene-by-Gene Typing System for Standardized High-Resolution Strain Typing of Bacillus anthracis

Journal article published in 2021 by Mostafa Y. Abdel-Glil ORCID, Alexandra Chiaverini ORCID, Giuliano Garofolo ORCID, Antonio Fasanella, Antonio Parisi, Dag Harmsen, Keith A. Jolley, Mandy C. Elschner, Herbert Tomaso, Jörg Linde, Domenico Galante ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Whole-genome sequencing (WGS) has been established for bacterial subtyping and is regularly used to study pathogen transmission, to investigate outbreaks, and to perform routine surveillance. Core-genome multilocus sequence typing (cgMLST) is a bacterial subtyping method that uses WGS data to provide a high-resolution strain characterization. This study aimed at developing a novel cgMLST scheme for Bacillus anthracis , a notorious pathogen that causes anthrax in livestock and humans worldwide.