Leptospirosis is a zoonosis caused by spirochetes of the genus Leptospira. It has a worldwide distribution with greater occurrence in tropical and subtropical countries. It is endemic in Brazil. It affects domestic, wild and production animals. The goal of this study was to assess dairy herd productive and reproductive indexes on a monthly basis by serologically monitoring the infection dynamics on two experimental groups: one with animals with negative results at study onset (G-1) and another with animals tested positive for at least one leptospira serovar (G-2). The serum microscopic agglutination test (MAT) was employed. Animals with titer equal to or greater than 100 IU were considered reactive. Animals were evaluated for productive and reproductive indexes based on data provided by the dairy’s IT system. Blood was collected from all animals in both groups once a month for nine months. Analysis showed interference between animals seroreactive to leptospirosis and both milk production and number of pregnancies for G-2 at collection moments 3, 4, 5, 6, 7 and 9 whereas for G-1 the same indexes showed decrease only in the 5th and 9th study months. The most prevalent serovars were Hardjoprajitino 59.5%, Pyrogenes 21.04%, Pomona 11.07%, Wollfi 11.07%, Hardjo 8.78%, Guaricura 6.55%, Copenhageni 5.09%, Icterohaemorrhagiae 1.11%, and Ctg 0.83%. Serovar Hardjoprajitino showed a relationship with herd milk production decrease.