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Published in

National Academy of Sciences, Proceedings of the National Academy of Sciences, 23(118), 2021

DOI: 10.1073/pnas.2015037118

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Generation of ordered protein assemblies using rigid three-body fusion

This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Significance Designed protein assemblies have many applications in medicine and technology. A long-standing design paradigm generates assemblies through genetic fusion of homo-oligomers via α-helical linkers. Here, we introduce a rigid fusion strategy that finds a greater number of design solutions and reduces formation of unintended assembly states, and a method for doubly anchoring target binding domains in the resulting assemblies that could contribute to cryogenic electron microscopy structure determination of small proteins.