Plants of the Daucus genus (Apiaceae) are used worldwide as traditional medicines or culinary ingredients. In this work, in order to explore a new chemotype, the essential oil obtained from the aerial parts of Daucus reboudii Coss., collected from the National Park of Gouraya (Bejaïa, Algeria), was analyzed by GC-MS. Twenty-eight compounds were identified, accounting for 96.6% of the total oil. (E)-anethol was the main constituent (59.4%), followed by estragol (21.2%) and dodecanal (4.4%). (E)-anethol is an uncommon constituent of Daucus genus, hence it could be considered as a marker that contributes to differentiating D. reboudii from other species. Metal chelating, ABTS•+ and DPPH• assays were performed to determine the antioxidant activity. The highest activity was revealed by the DPPH• method, where D. reboudii essential oil showed a significantly higher activity compared to the reference standard BHT at doses of 50 and 100 µg/mL. Results suggest that the essential oil from D. reboudii could have a potential use in the food industry as food preservative. Nevertheless, further studies are needed to assess its applicability, and to elucidate also the composition of non-volatile compounds of this plant.