SUMMARY This study aimed to evaluate the effects of bee pollen (BP) on the performance of laying Japanese quails and egg quality. A total of 60 quails were used in a completely randomized experimental design with four treatments (0%, 0.5%, 1%, and 1.5% BP inclusion in diets) and five replicates. At the end of the study, productive performance and egg quality were evaluated; 160 eggs were maintained at different temperatures based on a completely randomized design and factorial arrangement (4 × 2), with four BP levels (cited above) and two storage temperatures (room temperature and refrigeration) for 14 days, totaling eight treatments with four replicates. BP levels did not influence (P> 0.05) feed conversion; however, daily feed intake, laying rate, egg mass, and weight and percentage of albumen increased. Yolk percentage decreased due to the augmentation of BP in diets. There was no effect (P> 0.05) of interaction of storage temperature × BP levels on egg quality, except with regard to the Haugh unit. BP supplementation resulted in higher egg weight and index of yolk and albumen. Refrigerated eggs had better internal quality than normal eggs. BP inclusion in diets of Japanese quails improved bird performance and the quality of fresh and stored eggs.