Abstract This article explores the influence of different colors of lights on human physiological parameters and at the same time the effects on human emotions, and tries to explore the inner connection of the changes. It helps us to better understand the color of lighting and apply it in daily life or commercial purpose, Then the experience of visual sensation will be enhanced. This article examines the changes in physiological parameters and the subjective emotional ratings triggered by the six lighting conditions such as red, green, blue, warm white, cold white and darkness. From the experiment results, the changes of physiological parameters and subjective emotions can be observed under different lighting conditions, which can be applied to guide lighting design and projects.