This work contributes to the use of alternative adhesives in the wood-based industry, where pine wood is commonly used. The investigation identifies the influence and the optimal content (8, 12 and 15wt%) of a bio-based polyurethane adhesive in the production of medium density particleboards (MDP). A compaction pressure of 4 MPa at 100oC for 10 minutes is considered in the manufacture of panels based on pine wood residues and bio-based resin. The bulk density, flexural modulus (MOE) and strength (MOR) properties under static three-point bending are obtained according to the Brazilian standard NBR 14810. The results are compared with NBR 14810 and other standards to verify its performance based on the minimum requirements. Bulk density is not significantly affected by the investigated adhesive levels. MOE and MOR reach average values equivalent to 12wt% and 15wt% of the adhesive, and both meet the minimum requirements established in international normative documents. The adhesive level range responsible for maximizing ρ, MOE and MOR is between 12.42wt% and 15.79wt%.