Published in
Royal Society of Chemistry, Chemical Science, 9(12), p. 3194-3201, 2021
DOI: 10.1039/d0sc06001a
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A straightforward methodology to overcome solubility challenges for N-terminal cysteinyl peptide segments used in native chemical ligation
Journal article published in 2021 by
Skander A. Abboud,
El hadji Cisse,
Michel Doudeau,
Hélène Bénédetti,
Vincent Aucagne 
This paper is made freely available by the publisher.
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Abstract
We herein describe a straightforward approach for the introduction of a solubilizing tag on N-terminal cysteinyl segments used in native chemical ligation-based protein chemical synthesis. Conveniently, the tag is removed during the ligation.