Published in
American Institute of Physics, Applied Physics Letters, 22(101), p. 223704
DOI: 10.1063/1.4768929
Links
- ORCID
- American Institute of Physics
- [www.ncbi.nlm.nih.gov] | PDF
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- [europepmc.org] | PDF
- [www.researchgate.net] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
- [arxiv.org] | PDF
Tools
Studying DNA translocation in nanocapillaries using single molecule fluorescence
,
Sandip Ghosal,
Silvia Hernández-Ainsa,
Nicholas A. W. Bell,
Ulrich F. Keyser
Full text: Download
Abstract
We demonstrate simultaneous measurements of DNA translocation into glass nanopores using ionic current detection and fluorescent imaging. We verify the correspondence between the passage of a single DNA molecule through the nanopore and the accompanying characteristic ionic current blockage. By tracking the motion of individual DNA molecules in the nanocapillary perpendicular to the optical axis and using a model, we can extract an effective mobility constant for DNA in our geometry under high electric fields. ; Comment: 4 pages, 3 figures