Aim of study: To assess the potential of automated X-ray image analysis to evaluate the physical characteristics of Jatropha curcas seeds, and to relate the parameters obtained with the physiological quality of the seeds harvested at different maturity stages.Area of study: Experimental area of Agronomy Department, Federal University of Viçosa (UFV), Brazil.Material and methods: The fruits were harvested from 20 plants, based on the external skin color (green, yellow, brownish-yellow and brown). The study was performed by automated and visual analysis of radiographic images of the seeds, in which measurements of tissue integrity, density and seed filling were performed. Seed dry matter, germination and seedling growth were also analysed.Main results: Variables obtained through automated analysis of radiographic images correlated significantly with all physiological variables (r > 0.9), as well as visual image evaluations (r > 0.75). The seeds extracted from green fruits presented lower tissue integrity and lower physiological quality. Radiographic analysis was efficient for monitoring J. curcas seed quality at different maturity stages. Morpho-anatomical parameters obtained from X-ray analysis were highly correlated with seed physiological attributes.Research highlights: It is important to develop and improve methodologies based on lower-cost techniques, such as X-ray analysis. In this context, we verified that X-ray images can be used for monitoring J. curcas seed filling and maturation. Radiographic images of seeds can be analyzed automatically with ImageJ software. Internal morphology and physical characteristics of seeds have relationship with their physiological quality.